8GKK
Crystal Structure of the Humanized MUC16 Specific Antibody huAR9.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.736, 94.974, 110.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.140 - 1.750 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.19150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.867 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.140 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.515 |
Rpim | 0.031 | 0.236 |
Number of reflections | 436695 | 33472 |
<I/σ(I)> | 14.07 | |
Completeness [%] | 97.6 | |
Redundancy | 6.3 | |
CC(1/2) | 0.905 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis-Tris, pH 7.0 2.2 M ammonium sulfate |