8GKK

Crystal Structure of the Humanized MUC16 Specific Antibody huAR9.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	66.736, 94.974, 110.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.140 - 1.750
R-factor	0.1683
Rwork	0.167
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.140	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.070	0.515
Rpim	0.031	0.236
Number of reflections	436695	33472
<I/σ(I)>	14.07
Completeness [%]	97.6
Redundancy	6.3
CC(1/2)	0.905	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Bis-Tris, pH 7.0 2.2 M ammonium sulfate