8GK6

Crystal structure of extracellular domain of CNNM4 from Echinococcus granulosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.95371
Spacegroup name	P 1 21 1
Unit cell lengths	55.338, 120.764, 95.254
Unit cell angles	90.00, 98.09, 90.00

Refinement procedure

Resolution	46.640 - 2.700
R-factor	0.2011
Rwork	0.197
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.767
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.700	2.700
Rmerge	0.096	0.727
Number of reflections	45839	1246
<I/σ(I)>	14	1.2
Completeness [%]	97.4
Redundancy	4.7
CC(1/2)	0.990	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG 6000