8GJW

Structure of a cGAS-like receptor Cv-cGLR1 from C. virginica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	68.602, 68.602, 325.851
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.910 - 1.930
R-factor	0.2287
Rwork	0.228
R-free	0.24740
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	1.980
High resolution limit [Å]	1.930	1.930
Number of reflections	35645	2316
<I/σ(I)>	21.7
Completeness [%]	100.0
Redundancy	21.7
CC(1/2)	0.999	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M TRIS pH8.5, 20% w/v PEG4000, 0.2 M Li2SO4