8GJH
Salmonella ArnA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.078 |
Spacegroup name | P 1 |
Unit cell lengths | 90.826, 129.439, 140.188 |
Unit cell angles | 62.99, 71.66, 77.76 |
Refinement procedure
Resolution | 29.830 - 3.600 |
R-factor | 0.2194 |
Rwork | 0.218 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z7e |
RMSD bond length | 0.004 |
RMSD bond angle | 0.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rpim | 0.098 | |
Number of reflections | 59279 | 5929 |
<I/σ(I)> | 7 | |
Completeness [%] | 95.4 | 96 |
Redundancy | 1.5 | 1.5 |
CC(1/2) | 0.525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.5 to 2.0 M ammonium sulfate and 0.1M MES pH 6.75 |