8GJA

RAD51C-XRCC3 structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.11583
Spacegroup name	P 21 21 21
Unit cell lengths	65.940, 112.300, 259.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.330 - 2.600
R-factor	0.1871
Rwork	0.186
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.330	2.670
High resolution limit [Å]	2.600	2.600
Number of reflections	60211	4378
<I/σ(I)>	15.5
Completeness [%]	99.9
Redundancy	26.4
CC(1/2)	0.999	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	(25 mg/mL protein)+ 2.5 mM ADP601 BeF + 5mM MgCl2, grew with a 1:2 protein:crystallization reagent (1 M HEPES pH 7.5, 20% PEG 3,350, 2% Tacsimate pH 7.0,) ratio and reached maximum growth after 15 days.