8GJ8

RAD51C C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11587
Spacegroup name	P 32 2 1
Unit cell lengths	96.032, 96.032, 55.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.360 - 2.300
R-factor	0.19015
Rwork	0.188
R-free	0.23745
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.856
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.360	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	13402	1314
<I/σ(I)>	15.3
Completeness [%]	99.7
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	(25 mg/mL protein) crystallized in 1:1 protein:crystallization reagent (0.1 M Bis-TRIS pH 5.5, 0.3 M Na formate), with best diffraction achieved after additive screen optimization with 4% 1,3-propanediol and 10 days of growth.