8GJ8
RAD51C C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11587 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.032, 96.032, 55.683 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.360 - 2.300 |
R-factor | 0.19015 |
Rwork | 0.188 |
R-free | 0.23745 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.856 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.360 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 13402 | 1314 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 99.7 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | (25 mg/mL protein) crystallized in 1:1 protein:crystallization reagent (0.1 M Bis-TRIS pH 5.5, 0.3 M Na formate), with best diffraction achieved after additive screen optimization with 4% 1,3-propanediol and 10 days of growth. |