8GIR
Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.2mM Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.891 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.545, 98.506, 142.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.790 - 2.500 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.060 |
Data reduction software | XDS (20210205) |
Data scaling software | Aimless (0.7.7) |
Phasing software | MOLREP (11.7.03) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.093 | 0.642 |
Rmeas | 0.101 | 0.703 |
Rpim | 0.039 | 0.283 |
Total number of observations | 247600 | 24108 |
Number of reflections | 38237 | 4060 |
<I/σ(I)> | 11.8 | 2.5 |
Completeness [%] | 99.4 | |
Redundancy | 6.5 | 5.9 |
CC(1/2) | 0.998 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5 |