8GIE
Crystal structure of a designed single-component Plasmodium falciparum AMA1-RON2L insertion fusion immunogen 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.630, 62.830, 60.470 |
Unit cell angles | 90.00, 96.47, 90.00 |
Refinement procedure
Resolution | 19.220 - 1.850 |
R-factor | 0.1925 |
Rwork | 0.191 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.626 |
Data reduction software | XDS (v Jan 10, 2022) |
Data scaling software | XSCALE (v Jan 10, 2022) |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.220 | 1.916 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.100 | 0.676 |
Rmeas | 0.119 | 0.119 |
Rpim | 0.064 | 0.426 |
Number of reflections | 25370 | 2516 |
<I/σ(I)> | 10.61 | 2.32 |
Completeness [%] | 97.7 | 97.52 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.994 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.5 M Lithium Chloride, 0.1 M Tris (pH 8.5), 34% (w/v) PEG 6000 |