8GI6
Crystal structure of RhoA mutant L69R complexed with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.960, 64.187, 83.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.540 - 1.400 |
| R-factor | 0.15448 |
| Rwork | 0.153 |
| R-free | 0.18173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.031 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP (v11.0) |
| Refinement software | REFMAC (v5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.540 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.103 | 0.262 |
| Rmeas | 0.112 | 0.285 |
| Rpim | 0.044 | 0.110 |
| Number of reflections | 34454 | 3370 |
| <I/σ(I)> | 10.9 | 5.7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.993 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.7 | 293.15 | 0.1 M Na acetate (pH 5.7), 0.5 mM Na citrate, 26% PEG4000, 5% glycerol |
