8GEW
H-FABP crystal soaked in a bromo palmitic acid solution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.729320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.736, 54.966, 70.747 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.470 - 0.970 |
Rwork | 0.135 |
R-free | 0.15300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.894 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.410 | 0.990 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.051 | 0.847 |
Number of reflections | 77518 | |
<I/σ(I)> | 16.2 | 1.7 |
Completeness [%] | 99.5 | 98.4 |
Redundancy | 6.4 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM Tris pH 7.5, 20-25% polyethylene glycol (PEG) 4000 |