8GDT

Heligmosomoides polygyrus TGF-beta Mimic 6 Domain 3 (TGM6-D3) Bound to Human TGF-beta Type II Receptor Extracellular Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	56.042, 128.387, 29.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.253 - 1.401
Rwork	0.218
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1M9Z & 7SXB
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	1.420
High resolution limit [Å]	1.400	1.400
Rmerge		1.331
Rmeas	0.079	1.428
Rpim	0.022	0.506
Number of reflections	42468	1742
<I/σ(I)>	17.5	1.6
Completeness [%]	98.5	85.1
Redundancy	12.4	7.5
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M sodium cacodylate, 25% w/v PEG4000, pH 6.5