8GDL
Acid phosphatase pseudoenzyme from flea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.968, 71.898, 113.513 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 44.540 - 1.750 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.150 | 0.055 | 0.745 |
| Number of reflections | 75041 | 3817 | 3540 |
| <I/σ(I)> | 4.9 | ||
| Completeness [%] | 96.1 | 95.9 | |
| Redundancy | 5.9 | 5.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 3 M ammonium sulfate, 0.1 M MES pH 5.4 |
