8GCI
Crystal structure of C. elegans LIN-42 PAS-B domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 50.733, 105.907, 152.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.940 - 2.420 |
R-factor | 0.2657 |
Rwork | 0.263 |
R-free | 0.29290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gec |
RMSD bond length | 0.009 |
RMSD bond angle | 1.083 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.940 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.140 | 0.658 |
Number of reflections | 16123 | 1634 |
<I/σ(I)> | 10.2 | 3.3 |
Completeness [%] | 99.5 | |
Redundancy | 7.1 | |
CC(1/2) | 0.994 | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.15M Lithium sulfate, 1.5M sodium potassium tartrate |