8GC0
Crystal structure of PC39-50L, an anti-HIV broadly neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.810, 69.219, 81.643 |
Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
Resolution | 40.710 - 2.430 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.25190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.538 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.430 | 2.430 |
Rpim | 0.080 | 0.320 |
Number of reflections | 16654 | 761 |
<I/σ(I)> | 11.2 | 1.7 |
Completeness [%] | 98.3 | 90.3 |
Redundancy | 5.1 | 3.9 |
CC(1/2) | 0.880 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 0.085 M sodium acetate pH 4.6, 0.17 M ammonium acetate, 15% glycerol, 25.5% PEG 4000 |