8GBT
Time-resolve SFX structure of a photoproduct of carbon monoxide complex of bovine cytochrome c oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-06-16 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 178.300, 189.000, 209.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.002 - 2.800 |
| Rwork | 0.223 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.553 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.450 | 2.990 |
| High resolution limit [Å] | 1.759 | 2.870 |
| Number of reflections | 172414 | 17697 |
| <I/σ(I)> | 6.92 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.859 | 0.226 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.8 | 277 | PEG4000, Sodium Phosphate, decylmaltoside |
