8GBF
Crystal structure of human DNA polymerase eta incorporating syn-ITP across dT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2022-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 61 |
Unit cell lengths | 99.297, 99.297, 81.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.650 - 2.110 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.900 | 2.150 |
High resolution limit [Å] | 2.110 | 2.110 |
Rpim | 0.052 | |
Number of reflections | 25548 | 1501 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 96.7 | 93.24 |
Redundancy | 10.46 | 9.65 |
CC(1/2) | 0.997 | 0.489 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES, pH 6.25, 18% PEG2000 MME, 5 mM calcium chloride |