8G8V

GTP Cyclohydrolase-IB with sodium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	116.499, 116.499, 70.806
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.080 - 2.030
R-factor	0.2012
Rwork	0.199
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.669
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.080	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.237	1.719
Rmeas	0.243	1.771
Rpim	0.055	0.424
Number of reflections	36073	3546
<I/σ(I)>	13.5	1.67
Completeness [%]	99.9	99.77
Redundancy	19.8	17
CC(1/2)	0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES Na, 1.6 M ammonium sulfate, 2% PEG 1000