8G8S
bipartite p52 NLS in complex with Importin alpha 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.066, 88.583, 96.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.730 - 2.100 |
R-factor | 0.1696 |
Rwork | 0.169 |
R-free | 0.18610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bw0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.803 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.730 | 2.300 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 39376 | 1917 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 100.0 | |
Redundancy | 9.4 | |
CC(1/2) | 0.997 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.01M DTT, 0.1M sodium HEPES pH7, 0.6M sodium citrate |