8G8P

F420-2/GTP(GDP) complex of F420-gamma glutamyl ligase (CofE) from Archaeoglobus fulgidus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 41 2 2
Unit cell lengths	68.238, 68.238, 91.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.298 - 1.830
Rwork	0.197
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	1.880
High resolution limit [Å]	1.830	1.830
Rpim	0.039	0.333
Number of reflections	19796	990
<I/σ(I)>	13.7
Completeness [%]	100.0	100
Redundancy	13.7
CC(1/2)	0.998	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	0.8 M ammonium sulfate, 0.1 M citrate pH 4.5, 2 mM GTP, 5 mM Mn2+, 1 mM F420-2