8G8J

Crystal structure of human DNA polymerase eta incorporating ITP across dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	120
Detector technology	PIXEL
Collection date	2022-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97933
Spacegroup name	P 61
Unit cell lengths	98.900, 98.900, 81.222
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.820 - 1.740
R-factor	0.2132
Rwork	0.211
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.009
RMSD bond angle	1.176
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.936	1.768
High resolution limit [Å]	1.737	1.737
Number of reflections	39730	2461
<I/σ(I)>	15.35	1.2
Completeness [%]	86.0
Redundancy	11.96
CC(1/2)	0.999	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, pH 6.5, 20% PEG2000 MME, 5 mM calcium chloride