8G8C
Crystal structure of DH1322.1 Fab in complex with HIV proximal MPER peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.308, 182.950, 84.414 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.080 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.160 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.044 | 0.320 |
| Number of reflections | 65739 | 5860 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 25% PEG 1500 and 0.1M SPG buffer pH 8.5 |
