8G7R

Structure of the Escherichia coli 70S ribosome in complex with A-site tRNAIle(LAU) bound to the cognate AUA codon (Structure III)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	LEICA EM GP

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	FEI FALCON III (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.8
Software	cryoSPARC,PHENIX,UCSF Chimera
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION