8G64
Heme-bound flavodoxin FldH from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.991840 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.240, 67.240, 99.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.860 - 1.600 |
R-factor | 0.1605 |
Rwork | 0.160 |
R-free | 0.17830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.862 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.860 | 10.000 | 1.700 |
High resolution limit [Å] | 1.600 | 5.000 | 1.600 |
Rmerge | 0.063 | 0.038 | 0.787 |
Rmeas | 0.067 | 0.040 | 0.830 |
Number of reflections | 66287 | 1265 | 5678 |
<I/σ(I)> | 18.49 | ||
Completeness [%] | 99.6 | ||
Redundancy | 9.8 | ||
CC(1/2) | 0.999 | 0.999 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium citrate pH 5.5, 2 M ammonium sulphate |