8G52

Crystal structure of a bacterial TPAT family transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	42.695, 85.409, 73.686
Unit cell angles	90.00, 90.14, 90.00

Refinement procedure

Resolution	38.190 - 1.880
R-factor	0.1846
Rwork	0.183
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.190	1.947
High resolution limit [Å]	1.880	1.880
Number of reflections	42298	4283
<I/σ(I)>	16.43
Completeness [%]	98.1
Redundancy	4.2
CC(1/2)	0.999	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30 % (w/v) PEG 3000, 0.1 M Tris: HCl, pH 7,0.2 M sodium chloride