Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95364 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 61.725, 24.451, 300.191 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 44.220 - 2.490 |
| R-factor | 0.2096 |
| Rwork | 0.207 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.470 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.220 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.170 | |
| Rmeas | 0.192 | |
| Rpim | 0.088 | 0.496 |
| Number of reflections | 16351 | 1703 |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 99.2 | 94.5 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.989 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.2 M Calcium Acetate Hydrate, 0.1 M Sodium Cacodylate:HCl pH 6.5, 40% (v/v) PEG 300 |
