8G28
Crystal Structure of the C-terminal Fragment of AAA ATPase from Streptococcus pneumoniae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.532, 71.532, 151.435 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.180 - 1.300 |
| R-factor | 0.1213 |
| Rwork | 0.120 |
| R-free | 0.14300 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.082 | 1.512 |
| Rmeas | 0.084 | |
| Rpim | 0.017 | 0.331 |
| Number of reflections | 57219 | 2800 |
| <I/σ(I)> | 42.8 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 24.4 | 21.1 |
| CC(1/2) | 0.999 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Protein: 5.6 mg/ml, 0.25M Sodium chloride, 0.01M Tris pH 8.3; Screen: Classics II (F12), 0.2M Sodium chloride, 0.1M HEPES pH 7.5, 25% (w/v) PEG3350. |
