8G1N

Structure of Campylobacter concisus PglC I57M/Q175M Variant with modeled C-terminus

Replaces:  5W7L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.5498
Spacegroup name	P 32 2 1
Unit cell lengths	70.802, 70.802, 188.442
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.810 - 2.740
R-factor	0.2604
Rwork	0.259
R-free	0.29690
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.475
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.814	2.870
High resolution limit [Å]	2.740	2.740
Rmerge	0.097	1.033
Rpim	0.032	0.434
Number of reflections	27735	1941
<I/σ(I)>	21.4	2.5
Completeness [%]	100.0
Redundancy	18.1	11.8
CC(1/2)	1.000	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290.15	Bis-Tris 0.1 M, magnesium chloride hexahydrate 0.4 M, PEG 3350 23%