8G1B
Crystal structure of polyreactive 2G02 human Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.160, 59.440, 99.280 |
Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
Resolution | 69.330 - 1.670 |
R-factor | 0.1948 |
Rwork | 0.194 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.842 |
Data reduction software | iMOSFLM |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.330 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 92961 | 9082 |
<I/σ(I)> | 7.22 | |
Completeness [%] | 96.4 | |
Redundancy | 1.9 | |
CC(1/2) | 0.943 | 0.171 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 3350, 0.2M Magnesium formate |