8G0Q

Crystal structure of the yeast Ndc80:Nuf2 head region with a bound Dam1 segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 1 2
Unit cell lengths	129.997, 129.997, 216.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.860 - 3.220
R-factor	0.264
Rwork	0.263
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	xia2
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.900	3.540
High resolution limit [Å]	3.220	3.220
Rmerge	0.171	3.440
Rmeas	0.162	3.550
Rpim	0.021	0.418
Number of reflections	24938	1549
<I/σ(I)>	16.2
Completeness [%]	93.6	75.8
Redundancy	70.6
CC(1/2)	0.990	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.1 M ADA buffer pH 6.8