8FZW
Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2021-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.666, 58.666, 151.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.390 - 1.480 |
R-factor | 0.1433 |
Rwork | 0.143 |
R-free | 0.15740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.932 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.390 | 1.533 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.119 | 1.937 |
Rmeas | 0.126 | 2.035 |
Rpim | 0.039 | 0.618 |
Number of reflections | 45060 | 4434 |
<I/σ(I)> | 10.89 | 0.89 |
Completeness [%] | 99.9 | 99.95 |
Redundancy | 10.3 | 10.5 |
CC(1/2) | 0.998 | 0.572 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277 | 1 M L-sodium potassium tartrate with 0.1 M ADA buffer (pH 6-6.5) |