8FZV
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Ala-Ala linker variant, expressed with SUMO tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 162.557, 162.557, 56.877 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.390 - 3.290 |
| R-factor | 0.2839 |
| Rwork | 0.282 |
| R-free | 0.31410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.365 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.390 | 3.409 |
| High resolution limit [Å] | 3.290 | 3.292 |
| Rmerge | 0.337 | 3.593 |
| Number of reflections | 12820 | 515 |
| <I/σ(I)> | 6.02 | |
| Completeness [%] | 90.7 | |
| Redundancy | 7.9 | |
| CC(1/2) | 0.990 | 0.283 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 100 mM sodium acetate, pH 4.6, 2.0 M sodium formate |
