8FZV
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Ala-Ala linker variant, expressed with SUMO tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 162.557, 162.557, 56.877 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.390 - 3.290 |
R-factor | 0.2839 |
Rwork | 0.282 |
R-free | 0.31410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.365 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.390 | 3.409 |
High resolution limit [Å] | 3.290 | 3.292 |
Rmerge | 0.337 | 3.593 |
Number of reflections | 12820 | 515 |
<I/σ(I)> | 6.02 | |
Completeness [%] | 90.7 | |
Redundancy | 7.9 | |
CC(1/2) | 0.990 | 0.283 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 100 mM sodium acetate, pH 4.6, 2.0 M sodium formate |