8FZP
Crystal structure of polyreactive 43G10 mouse Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.240, 108.470, 115.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.150 - 1.630 |
R-factor | 0.2253 |
Rwork | 0.224 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.874 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.150 | 1.692 |
High resolution limit [Å] | 1.630 | 1.634 |
Number of reflections | 97569 | 9583 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 99.3 | |
Redundancy | 23 | |
CC(1/2) | 0.741 | 0.094 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 16% PEG 4000, 0.2M Ammonium sulfate, 0.1M HEPES pH 7.5, 10% Isopropanol |