8FZO

Crystal structure of polyreactive 338E6 mouse Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	40.920, 59.650, 82.250
Unit cell angles	90.00, 99.10, 90.00

Refinement procedure

Resolution	81.210 - 1.830
R-factor	0.1748
Rwork	0.172
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.885
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.210	1.895
High resolution limit [Å]	1.830	1.830
Number of reflections	33933	3289
<I/σ(I)>	21.6
Completeness [%]	97.4
Redundancy	32.1
CC(1/2)	0.903	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	29% PEG 3000, 0.1M Sodium Sulfate, 0.1M Tris pH 8.5