8FZM
Human importin alpha 3 in complex with Bimax2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.209, 91.610, 99.219 |
| Unit cell angles | 79.33, 81.04, 89.96 |
Refinement procedure
| Resolution | 24.850 - 3.000 |
| R-factor | 0.2312 |
| Rwork | 0.230 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bwb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.850 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.177 | 0.667 |
| Rpim | 0.133 | 0.535 |
| Number of reflections | 31010 | 4496 |
| <I/σ(I)> | 3.8 | 1.5 |
| Completeness [%] | 94.1 | |
| Redundancy | 2.4 | |
| CC(1/2) | 0.972 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M sodium HEPES, 0.78M sodium citrate, 1% Dithiothreitol |
