8FZ8
Structure of cytochrome P450sky2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.980, 97.850, 52.500 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 35.840 - 1.430 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.290 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.054 | 0.418 |
| Rmeas | 0.063 | 0.483 |
| Rpim | 0.030 | 0.238 |
| Total number of observations | 253881 | 12781 |
| Number of reflections | 64363 | 3208 |
| <I/σ(I)> | 11.9 | 2.6 |
| Completeness [%] | 96.6 | |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M ammonium acetate, 0.1 M Tris:HCl buffer pH 8.5, 25% w/v PEG 3350 |
