8FZ4
The von Willebrand factor A domain of Anthrax toxin receptor 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.244, 88.969, 59.779 |
Unit cell angles | 90.00, 128.13, 90.00 |
Refinement procedure
Resolution | 39.080 - 2.190 |
R-factor | 0.2193 |
Rwork | 0.217 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.471 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.080 | 2.268 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.090 | 0.862 |
Rmeas | 0.097 | 0.932 |
Rpim | 0.036 | 0.350 |
Number of reflections | 16416 | 1620 |
<I/σ(I)> | 10.8 | 2.38 |
Completeness [%] | 98.6 | 98.96 |
Redundancy | 6.9 | 7 |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 1.2 uL of 20 mg/mL protein in 12.5 mM Tris pH 8.8, 200mM KCl, 10mM MgCl2 combined with 1.2 uL of 0.1 M Glycine pH 9.5, 24% PEG 3350 |