8FXT
Escherichia coli periplasmic Glucose-Binding Protein glucose complex: Acrylodan conjugate attached at W183C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.587, 36.533, 79.905 |
Unit cell angles | 90.00, 124.13, 90.00 |
Refinement procedure
Resolution | 24.750 - 1.530 |
R-factor | 0.1568 |
Rwork | 0.155 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.288 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.060 | 0.292 |
Rpim | 0.025 | 0.131 |
Number of reflections | 43030 | 2019 |
<I/σ(I)> | 25.54 | 3.99 |
Completeness [%] | 98.8 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 20% PEG 3350 0.2M potassium thiocyanate |