8FXF
Crystal structure of the coiled-coil domain of TRIM56
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | I 41 |
Unit cell lengths | 136.679, 136.679, 65.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.655 - 2.800 |
Rwork | 0.219 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ug2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.967 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 14908 | 2153 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 99.4 | |
Redundancy | 3.7 | |
CC(1/2) | 0.999 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | 10% Glycerol, 5% PEG3000, 30%PEG400, 0.1M Hepes pH 7.5 |