8FXF

Crystal structure of the coiled-coil domain of TRIM56

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	I 41
Unit cell lengths	136.679, 136.679, 65.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.655 - 2.800
Rwork	0.219
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ug2
RMSD bond length	0.007
RMSD bond angle	1.967
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	14908	2153
<I/σ(I)>	9.1
Completeness [%]	99.4
Redundancy	3.7
CC(1/2)	0.999	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	10% Glycerol, 5% PEG3000, 30%PEG400, 0.1M Hepes pH 7.5