8FWP

Crystal Structure of CDC10 - CDC3 heterocomplex from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97718
Spacegroup name	P 43 2 2
Unit cell lengths	83.547, 83.547, 220.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.220
R-factor	0.192
Rwork	0.190
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.030	43.030	2.350
High resolution limit [Å]	2.220	6.590	2.220
Rmerge	0.297	0.067	3.570
Rmeas	0.303	0.069	3.637
Rpim	6.800		80.200
Number of reflections	39667	1702	6248
<I/σ(I)>	11.99		1.2
Completeness [%]	99.9
Redundancy	26
CC(1/2)	0.996	0.996	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283	0.2 M Sodium formate 0.1 M Bis-Tris propane 6.5 20 % w/v PEG 3350