8FWH
Crystal structure of bivalent antibody Fab fragment of Anti-human LAG3 (22D2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.287, 44.390, 89.084 |
| Unit cell angles | 90.00, 106.14, 90.00 |
Refinement procedure
| Resolution | 42.750 - 2.833 |
| Rwork | 0.216 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wkm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.570 | 2.900 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Number of reflections | 13587 | 13515 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 92.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.980 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M Tris-HCl, pH 7.5, 0.2 M sodium chloride, 10% w/v PEG8000 |
