8FWA
Phycocyanin structure from a modular droplet injector for serial femtosecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE MFX |
Synchrotron site | SLAC LCLS |
Beamline | MFX |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2021-05-29 |
Detector | SLAC ePix10k |
Wavelength(s) | 1.285 |
Spacegroup name | H 3 2 |
Unit cell lengths | 188.300, 188.300, 61.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.310 - 2.000 |
R-factor | 0.20632 |
Rwork | 0.204 |
R-free | 0.24512 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.143 |
Data reduction software | CrystFEL (0.10.1) |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 54596 | 5458 |
<I/σ(I)> | 1.8 | 0.6 |
Completeness [%] | 100.0 | |
Redundancy | 28 | |
CC(1/2) | 0.717 | 0.144 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277 | 75 mM HEPES pH 7.0, 20 mM MgCl2, 9% PEG 3350 |