8FVQ
PCSK9 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-02 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.300, 150.593, 63.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.540 - 2.570 |
| R-factor | 0.2041 |
| Rwork | 0.201 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | FFT |
| Refinement software | PHENIX (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 2.583 |
| High resolution limit [Å] | 2.570 | 2.574 |
| Rmerge | 0.150 | |
| Rmeas | 0.624 | |
| Number of reflections | 129813 | 1370 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20.0% Peg-6000, 0.1M Tris pH8.0 |
