8FVP
PCSK9 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-09 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.033, 70.407, 150.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.962 - 2.602 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.21460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | FFT |
| Refinement software | PHENIX (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.112 | 2.610 |
| High resolution limit [Å] | 2.602 | 2.602 |
| Rmerge | 0.101 | 0.629 |
| Number of reflections | 21242 | 204 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20.0% Peg-6000, 0.1M Tris pH8.0 |
