8FUD

Crystal structure of Vps29 in complex with Chaetomium thermophilum Vps5 (71 to 80)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953644
Spacegroup name	C 2 2 21
Unit cell lengths	54.051, 68.597, 211.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.620 - 1.680
R-factor	0.1782
Rwork	0.177
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.460	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.043	0.301
Rmeas	0.047	0.323
Rpim	0.017	0.116
Total number of observations	332485	15268
Number of reflections	44804	2057
<I/σ(I)>	18.6	4
Completeness [%]	98.9
Redundancy	7.4	7.4
CC(1/2)	0.999	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	40 mM KH2PO4, 16% PEG8000 and 5% glycerol