8FUB
Crystal structure of human Importin alpha 3 in complex with Hendra virus matrix protein NLS1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-25 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.060, 64.270, 158.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.780 - 2.750 |
R-factor | 0.243 |
Rwork | 0.241 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bvz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.570 |
Data reduction software | MOSFLM (1.14_3260) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.780 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.181 | 1.032 |
Rpim | 0.446 | |
Number of reflections | 13681 | 1966 |
<I/σ(I)> | 7.8 | 2.3 |
Completeness [%] | 99.9 | |
Redundancy | 11.3 | |
CC(1/2) | 0.995 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M sodium HEPES,0.72M sodium citrate, 1% dithiothreitol |