8FTY
Crystal structure of the carotenoid isomerooxygenase, NinaB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 349.934, 52.036, 210.036 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 49.670 - 1.950 |
| R-factor | 0.21891 |
| Rwork | 0.217 |
| R-free | 0.24677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.844 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.152 | 1.401 |
| Number of reflections | 271195 | 42167 |
| <I/σ(I)> | 5.6 | 0.92 |
| Completeness [%] | 98.6 | 95.7 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.996 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 40% (v/v) MPD 100 mM sodium cacodylate, pH 6.5 5% w/v PEG 8000 |
