8FT0

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2021-02-24
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.821, 77.821, 37.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.120 - 1.600
R-factor	0.2468
Rwork	0.244
R-free	0.29442
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.686
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.110	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.174	2.204
Rmeas	0.197	2.674
Rpim	0.087	1.477
Total number of observations	73492	2259
Number of reflections	15554	744
<I/σ(I)>	4.7	0.3
Completeness [%]	99.4
Redundancy	4.7	3
CC(1/2)	0.815	0.151

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection