8FSW

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2021-01-14
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.357, 77.357, 37.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.050 - 1.600
R-factor	0.22421
Rwork	0.222
R-free	0.26518
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.050	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.277	5.957
Rmeas	0.314	7.201
Rpim	0.141	3.971
Total number of observations	72224	2258
Number of reflections	15248	788
<I/σ(I)>	7.5	0.6
Completeness [%]	99.1
Redundancy	4.7	2.9
CC(1/2)	0.930	0.159

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection