8FSV

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-15
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.160, 77.160, 37.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.370 - 1.600
R-factor	0.20753
Rwork	0.205
R-free	0.24996
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.543
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.370	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.073	0.443
Rmeas	0.082	0.541
Rpim	0.035	0.305
Total number of observations	69982	2137
Number of reflections	15309	754
<I/σ(I)>	10	1.6
Completeness [%]	99.7
Redundancy	4.6	2.8
CC(1/2)	0.997	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection