8FSO

Crystal structure of integrin beta-6 tail bound to the FERM-folded talin head domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	66.777, 67.347, 115.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.090 - 2.330
R-factor	0.2098
Rwork	0.208
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.894
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.400
High resolution limit [Å]	2.330	2.330
Rmerge	0.074	0.740
Number of reflections	22692	1620
<I/σ(I)>	14.4
Completeness [%]	99.7
Redundancy	3.5
CC(1/2)	0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	PEG3350 20%, NaCl 0.1M, DTT 2mM